Rabbit anti-Phospho RelA (S468) Antibody, Affinity Purified

Name	Rabbit anti-Phospho RelA (S468) Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0237
Prices	$386.00
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Pig, Ape, Human, Horse, Mouse, Guinea Pig, Primate, Monkey, Bovine, Dog, Rat
Gene	RELA
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-Phospho RelA (S468) Antibody, Affinity Purified: <B>Cat. No. PLA0237</B>: Detection of Mouse Phospho RelA (S468) by Immunohistochemistry. Samples: FFPE sections of Mouse renal cell carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified Rabbit Anti-Phospho RelA (S468) (<B>Cat. No. PLA0237</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-Phospho RelA (S468) Antibody, Affinity Purified: Cat. No. PLA0237: Detection of Mouse Phospho RelA (S468) by Immunohistochemistry. Samples: FFPE sections of Mouse renal cell carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified Rabbit Anti-Phospho RelA (S468) (Cat. No. PLA0237) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

<B>Immunohistochemistry</B><BR/>Rabbit Anti-Phospho RelA (S468) Antibody, Affinity Purified: <B>Cat. No. PLA0237</B>: Detection of Human Phospho RelA (S468) by Immunohistochemistry. Samples: FFPE sections of Human ovarian carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified Rabbit Anti-Phospho RelA (S468) (<B>Cat. No. PLA0237</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-Phospho RelA (S468) Antibody, Affinity Purified: Cat. No. PLA0237: Detection of Human Phospho RelA (S468) by Immunohistochemistry. Samples: FFPE sections of Human ovarian carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified Rabbit Anti-Phospho RelA (S468) (Cat. No. PLA0237) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-Phospho RelA (S468) Antibody, Affinity Purified: <B>Cat. No. PLA0237</B>: Detection of Human Phospho RelA (S468) by Western Blot. Samples: Whole cell lysate (50 μg) from Jurkat cells treated with TNF alpha and Calyculin A (+) or mock treated (-). Antibodies: Affinity purified Rabbit Anti-Phospho RelA (S468) Antibody <B>PLA0237</B> used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunoblotting
Rabbit Anti-Phospho RelA (S468) Antibody, Affinity Purified: Cat. No. PLA0237: Detection of Human Phospho RelA (S468) by Western Blot. Samples: Whole cell lysate (50 μg) from Jurkat cells treated with TNF alpha and Calyculin A (+) or mock treated (-). Antibodies: Affinity purified Rabbit Anti-Phospho RelA (S468) Antibody PLA0237 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-Phospho RelA (S468) Antibody, Affinity Purified: <B>Cat. No. PLA0237</B>: Detection of Human Phospho RelA (S468) by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells treated with TNF alpha and Calyculin A (+) or mock treated (-); Immunoprecipitates treated (+) or untreated (-) with phosphatase. Antibodies: RelA Antibody used at 6 μg/1mg lysate to immunoprecipitate total RelA protein. Affinity purified Rabbit Anti-Phospho RelA (S468) antibody <B>Cat. No. PLA0237</B> used for WB at 1.0 μg/mL to detect phospho RelA (S468) in immunoprecipitates (top panel); Affinity purified Rabbit Anti-RelA antibody used at 0.2 μg/mL to detect total RelA in immunoprecipitates (bottom). Detection: Chemiluminescence with exposure times of 10 seconds (top) and 30 seconds (bottom).

Immunoprecipitation
Rabbit Anti-Phospho RelA (S468) Antibody, Affinity Purified: Cat. No. PLA0237: Detection of Human Phospho RelA (S468) by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells treated with TNF alpha and Calyculin A (+) or mock treated (-); Immunoprecipitates treated (+) or untreated (-) with phosphatase. Antibodies: RelA Antibody used at 6 μg/1mg lysate to immunoprecipitate total RelA protein. Affinity purified Rabbit Anti-Phospho RelA (S468) antibody Cat. No. PLA0237 used for WB at 1.0 μg/mL to detect phospho RelA (S468) in immunoprecipitates (top panel); Affinity purified Rabbit Anti-RelA antibody used at 0.2 μg/mL to detect total RelA in immunoprecipitates (bottom). Detection: Chemiluminescence with exposure times of 10 seconds (top) and 30 seconds (bottom).