Rabbit anti-Phospho MCM2 (S27) Antibody, Affinity Purified

Name	Rabbit anti-Phospho MCM2 (S27) Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0033
Prices	$345.00
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Rat, Dog, Horse, Rabbit, Ape, Primate, Chimpanzee, Human, Mouse, Guinea Pig, Pig
Gene	MCM2
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-Phospho MCM2 (S27) Antibody, Affinity Purified: <B>Cat. No. PLA0033</B>: Detection of Human and Mouse Phospho MCM2 (S27) by Immunohistochemistry. Sample: FFPE sections of Human lung carcinoma (left) and Mouse renal cell carcinoma (right). Antibody: Affinity purified Rabbit Anti-phospho MCM2 (S27) (<B>Cat. No. PLA0033</B>) used at a dilution of 1:1,000 (1 μg/mL) and 1:5,000 (0.2 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-Phospho MCM2 (S27) Antibody, Affinity Purified: Cat. No. PLA0033: Detection of Human and Mouse Phospho MCM2 (S27) by Immunohistochemistry. Sample: FFPE sections of Human lung carcinoma (left) and Mouse renal cell carcinoma (right). Antibody: Affinity purified Rabbit Anti-phospho MCM2 (S27) (Cat. No. PLA0033) used at a dilution of 1:1,000 (1 μg/mL) and 1:5,000 (0.2 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-Phospho MCM2 (S27) Antibody, Affinity Purified: <B>Cat. No. PLA0033</B>: Detection of Human Phospho MCM2 (S27) by Western Blot. Samples: Whole cell lysate (30 μg) from HeLa cells that were either untreated or treated with alkaline phosphatase (AP). Antibodies: Affinity purified Rabbit Anti-phospho MCM2 (Ser27) Antibody <B>PLA0033</B> used at 0.07 μg/mL. After probing with the phospho-specific Antibodies, Rabbit Anti-MCM2 Antibody <B>PLA0060</B> was used at 0.2 μg/mL to show total MCM2. Detection: Chemiluminescence with a 1 minute exposure.

Immunoblotting
Rabbit Anti-Phospho MCM2 (S27) Antibody, Affinity Purified: Cat. No. PLA0033: Detection of Human Phospho MCM2 (S27) by Western Blot. Samples: Whole cell lysate (30 μg) from HeLa cells that were either untreated or treated with alkaline phosphatase (AP). Antibodies: Affinity purified Rabbit Anti-phospho MCM2 (Ser27) Antibody PLA0033 used at 0.07 μg/mL. After probing with the phospho-specific Antibodies, Rabbit Anti-MCM2 Antibody PLA0060 was used at 0.2 μg/mL to show total MCM2. Detection: Chemiluminescence with a 1 minute exposure.