Rabbit anti-Phospho MCM2 (S53) Antibody, Affinity Purified

Name	Rabbit anti-Phospho MCM2 (S53) Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0138
Prices	$345.00
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Mouse, Horse, Bovine, Turkey, Guinea Pig, Pig, Human, Zebrafish, Dog, Rat, Chicken, Rabbit
Gene	MCM2
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-Phospho MCM2 (S53) Antibody, Affinity Purified: <B>Cat. No. PLA0138</B>: Detection of Human and Mouse Phospho MCM2 (S53) by Immunohistochemistry. Sample: FFPE sections of Human breast carcinoma (left) and Mouse renal cell carcinoma (right). Antibody: Affinity purified Rabbit Anti-phospho MCM2 (S53) (<B>Cat. No. PLA0138</B>) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-Phospho MCM2 (S53) Antibody, Affinity Purified: Cat. No. PLA0138: Detection of Human and Mouse Phospho MCM2 (S53) by Immunohistochemistry. Sample: FFPE sections of Human breast carcinoma (left) and Mouse renal cell carcinoma (right). Antibody: Affinity purified Rabbit Anti-phospho MCM2 (S53) (Cat. No. PLA0138) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-Phospho MCM2 (S53) Antibody, Affinity Purified: <B>Cat. No. PLA0138</B>: Detection of Human Phospho MCM2 (S53) by Western Blot. Samples: Whole cell lysate (5 and 15 μg for WB; 1 mg for IP, 20% of IP loaded) from asynchronous 293T cells. For IP/WB, MCM2 was immunoprecipitated using <B>PLA0060</B>, which recognizes total MCM2. The immunoprecipitate was either mock treated (-) or treated (+) with phosphatase (PPase). Antibody: Affinity purified Rabbit Anti-phospho MCM2 (S53) Antibody <B>PLA0138</B> used at 0.1 μg/mL. To examine total MCM2, the membrane was stripped and blotted with <B>PLA0060</B> at 0.1 μg/mL. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Immunoblotting
Rabbit Anti-Phospho MCM2 (S53) Antibody, Affinity Purified: Cat. No. PLA0138: Detection of Human Phospho MCM2 (S53) by Western Blot. Samples: Whole cell lysate (5 and 15 μg for WB; 1 mg for IP, 20% of IP loaded) from asynchronous 293T cells. For IP/WB, MCM2 was immunoprecipitated using PLA0060, which recognizes total MCM2. The immunoprecipitate was either mock treated (-) or treated (+) with phosphatase (PPase). Antibody: Affinity purified Rabbit Anti-phospho MCM2 (S53) Antibody PLA0138 used at 0.1 μg/mL. To examine total MCM2, the membrane was stripped and blotted with PLA0060 at 0.1 μg/mL. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).