Rabbit anti-Vimentin Antibody, Affinity Purified

Name	Rabbit anti-Vimentin Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0199
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Rat, Sheep, Horse, Human, Chicken, Dog, Mouse, Rabbit, Pig, Bovine, Guinea Pig, Turkey
Gene	VIM
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-Vimentin Antibody, Affinity Purified: <B>Cat. No. PLA0199</B>: Detection of Human Vimentin by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-Vimentin (<B>Cat. No. PLA0199</B>) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-Vimentin Antibody, Affinity Purified: Cat. No. PLA0199: Detection of Human Vimentin by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-Vimentin (Cat. No. PLA0199) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-Vimentin Antibody, Affinity Purified: <B>Cat. No. PLA0199</B>: Detection of Human and Mouse Vimentin by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg), 293T (Lane T; 50 μg), and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibody: Affinity purified Rabbit Anti-Vimentin Antibody <B>PLA0199</B> used for WB at 0.04 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunoblotting
Rabbit Anti-Vimentin Antibody, Affinity Purified: Cat. No. PLA0199: Detection of Human and Mouse Vimentin by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg), 293T (Lane T; 50 μg), and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibody: Affinity purified Rabbit Anti-Vimentin Antibody PLA0199 used for WB at 0.04 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.