Rabbit anti-RPA32 Antibody, Affinity Purified

Name	Rabbit anti-RPA32 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0070
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Rabbit, Chimpanzee, Ape, Human, Horse, Pig, Marmoset, Monkey, Mouse
Gene	RPA2
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-RPA32 Antibody, Affinity Purified: <B>Cat. No. PLA0070</B>: Detection of Human RPA32 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-RPA32 (<B>Cat. No. PLA0070</B>) used at a dilution of 1:5,000 (0.2 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-RPA32 Antibody, Affinity Purified: Cat. No. PLA0070: Detection of Human RPA32 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-RPA32 (Cat. No. PLA0070) used at a dilution of 1:5,000 (0.2 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-RPA32 Antibody, Affinity Purified: <B>Cat. No. PLA0070</B>: Detection of Human and Mouse RPA32 by Western Blot. Samples: Whole cell lysate (50 μg) from 293T, HeLa, Jurkat, Mouse TCMK-1, and Mouse NIH3T3 cells. Antibodies: Affinity purified Rabbit Anti-RPA32 Antibody <B>PLA0070</B> used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunoblotting
Rabbit Anti-RPA32 Antibody, Affinity Purified: Cat. No. PLA0070: Detection of Human and Mouse RPA32 by Western Blot. Samples: Whole cell lysate (50 μg) from 293T, HeLa, Jurkat, Mouse TCMK-1, and Mouse NIH3T3 cells. Antibodies: Affinity purified Rabbit Anti-RPA32 Antibody PLA0070 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-RPA32 Antibody, Affinity Purified: <B>Cat. No. PLA0070</B>: Detection of Human RPA32 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from 293T cells. Antibodies: Affinity purified Rabbit Anti-RPA32 antibody <B>Cat. No. PLA0070</B> used for IP at 6 μg/mg lysate. RPA32 was inefficiently immunoprecipitated by Rabbit Anti-RPA32 antibody B. For blotting immunoprecipitated RPA32, <B>PLA0070</B> was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunoprecipitation
Rabbit Anti-RPA32 Antibody, Affinity Purified: Cat. No. PLA0070: Detection of Human RPA32 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from 293T cells. Antibodies: Affinity purified Rabbit Anti-RPA32 antibody Cat. No. PLA0070 used for IP at 6 μg/mg lysate. RPA32 was inefficiently immunoprecipitated by Rabbit Anti-RPA32 antibody B. For blotting immunoprecipitated RPA32, PLA0070 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.