Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-RFC4 Antibody, Affinity Purified:
Cat. No. PLA0090: Detection of Human RFC4 (aka RFC37) by Western Blot and Immunoprecipitation. Sample: Nuclear extract (20 μg for WB; 300 μg for IP) from HeLa cells. Antibody: Affinity purified Rabbit Anti-RFC4 (
Cat. No. PLA0090) used at 0.5 μg/mL for WB and 2 μg/300 μg lysate for IP. Detection: Chemiluminescence with 10 second exposure.