Rabbit anti-Phospho RNA Polymerase II (S2) Antibody, Affinity Purified

Name	Rabbit anti-Phospho RNA Polymerase II (S2) Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0128
Prices	$345.00
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Amphibians, Dog, Rabbit, Human, Zebrafish, Mouse, Rat, Primate, Monkey, Bovine, Drosophila, Pig
Gene	POLR2A
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-Phospho RNA Polymerase II (S2) Antibody, Affinity Purified: <B>Cat. No. PLA0128</B>: Detection of Human Phospho-RNA Polymerase II (S2) by Immunohistochemistry. Samples: FFPE serial sections of Human ovarian carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified Rabbit Anti-Phospho-RNA Polymerase II (S2) (<B>Cat. No. PLA0128</B>) used at a dilution of 1:500 ( 0.4 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-Phospho RNA Polymerase II (S2) Antibody, Affinity Purified: Cat. No. PLA0128: Detection of Human Phospho-RNA Polymerase II (S2) by Immunohistochemistry. Samples: FFPE serial sections of Human ovarian carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified Rabbit Anti-Phospho-RNA Polymerase II (S2) (Cat. No. PLA0128) used at a dilution of 1:500 ( 0.4 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-Phospho RNA Polymerase II (S2) Antibody, Affinity Purified: <B>Cat. No. PLA0128</B>: Detection of Phospho RNA Polymerase II (S2) in Whole Cell Lysates and Immunoprecipitates. Samples: Whole cell lysate (50 μg for A and Input for B; 1 mg for IP, 20% of IP loaded) from HeLa cells. In B, immunoprecipitates were either mock treated or treated with lambda phosphatase. Antibody: Affinity purified Rabbit Anti-Phospho RNA Polymerase II (S2) Antibody (<B>Cat. No. PLA0128</B>) used for WB at 0.02 μg/mL (A) and 0.1 μg/mL (B). In A, Antibody was pre-incubated without peptide (-), with nonphosphorylated peptide (NP), with phosphorylated S2 peptide, or with phosphorylated S5 peptide. In B, RNA Polymerase II was immunoprecipitated using Rabbit Anti-RNA Polymerase II Antibody (<B>Cat. No. PLA0127</B>) at 3 μg/mg lysate. Immunoprecipitates were either mock treated or treated with lambda phosphatase. Detection: Chemiluminescence with a exposure times of 10 seconds.

Immunoblotting
Rabbit Anti-Phospho RNA Polymerase II (S2) Antibody, Affinity Purified: Cat. No. PLA0128: Detection of Phospho RNA Polymerase II (S2) in Whole Cell Lysates and Immunoprecipitates. Samples: Whole cell lysate (50 μg for A and Input for B; 1 mg for IP, 20% of IP loaded) from HeLa cells. In B, immunoprecipitates were either mock treated or treated with lambda phosphatase. Antibody: Affinity purified Rabbit Anti-Phospho RNA Polymerase II (S2) Antibody (Cat. No. PLA0128) used for WB at 0.02 μg/mL (A) and 0.1 μg/mL (B). In A, Antibody was pre-incubated without peptide (-), with nonphosphorylated peptide (NP), with phosphorylated S2 peptide, or with phosphorylated S5 peptide. In B, RNA Polymerase II was immunoprecipitated using Rabbit Anti-RNA Polymerase II Antibody (Cat. No. PLA0127) at 3 μg/mg lysate. Immunoprecipitates were either mock treated or treated with lambda phosphatase. Detection: Chemiluminescence with a exposure times of 10 seconds.

<B>Immunoblotting</B><BR/>Rabbit Anti-Phospho RNA Polymerase II (S2) Antibody, Affinity Purified: <B>Cat. No. PLA0128</B>: Detection of Mouse Phospho RNA Polymerase II (S2) by Western Blot. Samples: Whole cell lysate (15 and 50 μg) from NIH3T3 cells. Antibodies: Affinity purified Rabbit Anti-Phospho RNA Polymerase II (S2) Antibody <B>PLA0128</B> used for WB at 0.08 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunoblotting
Rabbit Anti-Phospho RNA Polymerase II (S2) Antibody, Affinity Purified: Cat. No. PLA0128: Detection of Mouse Phospho RNA Polymerase II (S2) by Western Blot. Samples: Whole cell lysate (15 and 50 μg) from NIH3T3 cells. Antibodies: Affinity purified Rabbit Anti-Phospho RNA Polymerase II (S2) Antibody PLA0128 used for WB at 0.08 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.