Rabbit anti-BLM Antibody, Affinity Purified

Name	Rabbit anti-BLM Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0029
Prices	$323.50
Sizes	100 µl
Applications	IP
Species Reactivities	Bovine, Ape, Pig, Primate, Chimpanzee, Monkey, Human, Mouse, Dog
Gene	BLM
Supplier Page	Shop

Product images

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-BLM Antibody, Affinity Purified: <B>Cat. No. PLA0029</B>: Detection of Human BLM by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified Rabbit Anti-BLM Antibody <B>PLA0029</B> used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoblotting
Rabbit Anti-BLM Antibody, Affinity Purified: Cat. No. PLA0029: Detection of Human BLM by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified Rabbit Anti-BLM Antibody PLA0029 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

<B>Immunoblotting</B><BR/>Rabbit Anti-BLM Antibody, Affinity Purified: <B>Cat. No. PLA0029</B>: Detection of Mouse BLM by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified Rabbit Anti-BLM Antibody <B>PLA0029</B> used for WB at 2 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoblotting
Rabbit Anti-BLM Antibody, Affinity Purified: Cat. No. PLA0029: Detection of Mouse BLM by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified Rabbit Anti-BLM Antibody PLA0029 used for WB at 2 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-BLM Antibody, Affinity Purified: <B>Cat. No. PLA0029</B>: Detection of Human BLM by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BLM antibody <B>Cat. No. PLA0029</B> used for IP at 6 μg/mg lysate. BLM was also immunoprecipitated by a BLM Antibody B and C. For blotting immunoprecipitated BLM <B>PLA0029</B> was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation
Rabbit Anti-BLM Antibody, Affinity Purified: Cat. No. PLA0029: Detection of Human BLM by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BLM antibody Cat. No. PLA0029 used for IP at 6 μg/mg lysate. BLM was also immunoprecipitated by a BLM Antibody B and C. For blotting immunoprecipitated BLM PLA0029 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.