![Example of a typical JNK1/2 (pT183/Y185) cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/2196_ab176645-204189-JNK12T183Y185Fig1.jpg)
Example of a typical JNK1/2 (pT183/Y185) cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.
![Example of a typical JNK1/2 (pT183/Y185) recombinant protein standard curve. The proportion of total protein that is phosphorylated is unknown - data is indicative only. Background-subtracted data values (mean +/- SD) are graphed.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/2197_ab176645-204190-JNK12T183Y185Fig2.jpg)
Example of a typical JNK1/2 (pT183/Y185) recombinant protein standard curve. The proportion of total protein that is phosphorylated is unknown - data is indicative only. Background-subtracted data values (mean +/- SD) are graphed.
![Linearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1 x Cell Extraction Buffer PTR. Data from duplicate measurements of JNK1/2 (pT183/Y185) are normalized and plotted.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/2198_ab176645-204191-JNK12T183Y185Fig3.jpg)
Linearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1 x Cell Extraction Buffer PTR. Data from duplicate measurements of JNK1/2 (pT183/Y185) are normalized and plotted.
![Cell line analysis for Total JNK1/2 from 200 µg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/2199_ab176645-204192-JNK12T183Y185Fig4.jpg)
Cell line analysis for Total JNK1/2 from 200 µg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).
![Induction of JNK1/2 (pT183/Y185) phosphorylation in HeLa cells in response to anisomycin treatment. HeLa cells were cultured in 96-well tissue culture plates and treated (30 min) with a dose-range of anisomycin before cell lysis. Data from quadruplicate measurements of JNK1/2 (pT183/Y185) are plotted and compared against Total JNK1/2 protein levels. Comparative JNK1/2 (pT183/Y185) and JNK1/2 (Total) data also shown by Western Blot.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/2200_ab176645-204193-JNK12T183Y185Fig5.jpg)
Induction of JNK1/2 (pT183/Y185) phosphorylation in HeLa cells in response to anisomycin treatment. HeLa cells were cultured in 96-well tissue culture plates and treated (30 min) with a dose-range of anisomycin before cell lysis. Data from quadruplicate measurements of JNK1/2 (pT183/Y185) are plotted and compared against Total JNK1/2 protein levels. Comparative JNK1/2 (pT183/Y185) and JNK1/2 (Total) data also shown by Western Blot.