![Example of a typical JNK1/2 cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/2212_ab176646-204071-JNK12totalFig1.jpg)
Example of a typical JNK1/2 cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.
![Example of a typical JNK1/2 recombinant protein standard curve. Background-subtracted data values (mean +/- SD) are graphed.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/2213_ab176646-204072-JNK12totalFig2.jpg)
Example of a typical JNK1/2 recombinant protein standard curve. Background-subtracted data values (mean +/- SD) are graphed.
![Linearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1X Cell Extraction Buffer PTR. Data from duplicate measurements of JNK1/2 are normalized and plotted.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/2214_ab176646-204073-JNK12totalFig3.jpg)
Linearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1X Cell Extraction Buffer PTR. Data from duplicate measurements of JNK1/2 are normalized and plotted.
![Cell line analysis for Total JNK1/2 from 200 µg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/2215_ab176646-204075-JNK12totalFig4.jpg)
Cell line analysis for Total JNK1/2 from 200 µg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).
![Induction of JNK1/2 (pT183/Y185) phosphorylation in HeLa cells in response to anisomycin treatment. HeLa cells were cultured in 96-well tissue culture plates and treated (30 min) with a dose-range of anisomycin before cell lysis. Data from quadruplicate measurements of JNK1/2 (pT183/Y185) are plotted and compared against Total JNK1/2 protein levels. Comparative JNK1/2 (pT183/Y185) and JNK1/2 (Total) data also shown by Western Blot.](http://www.bioprodhub.com/system/product_images/ak_products/2/sub_1/2216_ab176646-204076-JNK12totalFig5.jpg)
Induction of JNK1/2 (pT183/Y185) phosphorylation in HeLa cells in response to anisomycin treatment. HeLa cells were cultured in 96-well tissue culture plates and treated (30 min) with a dose-range of anisomycin before cell lysis. Data from quadruplicate measurements of JNK1/2 (pT183/Y185) are plotted and compared against Total JNK1/2 protein levels. Comparative JNK1/2 (pT183/Y185) and JNK1/2 (Total) data also shown by Western Blot.