Background-subtracted data values (mean +/- SD) are graphed.
Background-subtracted data values (mean +/- SD, n=3) are graphed.
5, 25 and 50 µg/mL of lysates prepared from untreated HeLa cells, and MCF7 cells treated for 18 hours with 1 µM camptothecin or vehicle (DMSO) were analyzed using the ELISA kit. Background-subtracted data values (mean +/-SD, n=2-5) are graphed (in A). Interpolated p21 concentrations normalized to the total protein in the analyzed sample are graphed (in B).
20 µg of cell lysates prepared from MCF7 cells treated for 18 hours with 1 µM camptothecin (lane 2) or vehicle (DMSO, lane 1) or from untreated HeLa cells (lane 3) were analyzed by Western blotting using the p21 detector (in A) and the p21 capture (in B) antibodies of the ELISA kit. Membranes were re-probed with GAPDH antibody (ab110305) as a loading control.