Background-subtracted data values (mean +/- SD) are graphed.
Background-subtracted data values (mean +/- SD) are graphed.
Background-subtracted data values (mean +/- SD, n = 2) are graphed.
Jurkat cell lysates corresponding to 2x106 cells/mL or 1x106 cells/mL were prepared as previously described. The concentrations of Pro Caspase 3 were interpolated from data values shown in the previous figure using Pro Caspase 3 standard curve and graphed in ng of Pro Caspase 3 per 106 cells. Staurosporine IC50 determined from the interpolated data values were 0.70 µM and 0.56 µM using, respectively, lysates of 2x106 cells/mL and 1x106 cells/mL.
Jurkat cell lysates corresponding to 2x106 cells/mL or 1x106 cells/mL were prepared by direct in-well lysis (without media removal) from cells grown in RPMI1640 media supplemented with 10% FBS and treated for 4 hours with variable doses of staurosporine in a 96-well plate. Background-subtracted data values (mean +/- SD, n=3) are graphed. Staurosporine IC50 determined from background-subtracted data values were 0.75 µM and 0.57 µM using, respectively, lysates of 2x106 cells/mL and 1x106 cells/mL.