Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of sTNF RI were measured in duplicate and interpolated from the sTNF RI standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RI concentration was determined to be 3,427 pg/mL in mouse serum, 2,484 pg/mL in mouse plasma (Citrate), and 2,982 pg/mL in mouse plasma (EDTA).
The concentrations of sTNF RI were measured in duplicate and interpolated from the sTNF RI standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RI concentration was determined to be 1,754 pg/mL in mouse urine, 538.3 pg/mL L929 stimulated cell culture supernatant.
L929 and J774A.1 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. sTNF RI was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and J774A.1 and cell culture media. Measured values were interpolated from the sTNF RI Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed. sTNF RI is undetectable in media.