Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of sTNF RII were measured in duplicate and interpolated from the sTNF RII standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RII concentration was determined to be 12 ng/mL in serum, 13.2 ng/mL in plasma (Citrate), and 12.8 ng/mL plasma (EDTA).
The concentrations of sTNF RII were measured in duplicate and interpolated from the sTNF RII standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RII concentration was determined to be 13.3 ng/mL in urine and 3.9 ng/mL in RAW264.7 stimulated cell culture supernatant.
Cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. sTNF RII was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated RAW264.7, L929, an J774A.1 and cell culture media. Measured values were interpolated from the sTNF RII Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed. sTNF RII was undetectable in media.