Fluorescent analysis showing cells that are live (blue, stained by CytoCalcein Violet 450), apoptotic (green, Apopxin Green Indicator), and necrotic (red, indicated by 7-AAD staining) in Jurkat cells induced by 1μM staurosporine for 3 hours. The fluorescence images of the cells were taken with a fluorescent microscope through the Violet, FITC and TRITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown.
Jurkat cells were treated without (Blue) or with 1 μM staurosporine (Red) in a 37 oC, 5% CO2 incubator for 5 hours, and thenloaded with Apopxin Green Indicator for 30 minutes. The fluorescence intensity of Apopxin Green Indicator was measured with aflow cytometer using FL1 channel.
Fluorescent analysis of live non-induced Jurkat cells stained by CytoCalcein Violet 450.