PathScan ® Phospho-ALK (Tyr1604) Sandwich ELISA Kit
| Name | PathScan ® Phospho-ALK (Tyr1604) Sandwich ELISA Kit |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 7324 |
| Category | ELISA Kit |
| Prices | $489.00 |
| Sizes | 1 kit (96 assays) |
| Assay Type | Sandwich |
| Applications | ELISA |
| Species Reactivities | Human |
| Description | CST's PathScan ® Phospho-ALK (Tyr1604) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of phospho-ALK (Tyr1604) or phospho-NPM-ALK fusion protein |
| Gene | ALK |
| Supplier Page | Shop |
Product images
Figure 1: Constitutive phosphorylation of NPM-ALK in Karpas299 cells lysed in the presence of phosphatase inhibitors (phospho-lysate) can be detected by PathScan ® Phospho-ALK (Tyr1604) Sandwich ELISA Kit #7324. In contrast, only a low level of phospho-NPM-ALK protein is detected in Karpas299 cells lysed without addition of phosphatase inhibitors to the lysis buffer (nonphospho-lysate). However, similar levels of total NPM-ALK protein from either nonphospho or phospho-lysates can be detected by PathScan ® Total ALK Sandwich ELISA Kit #7322. Absorbance at 450 nm is shown in the top figure, while the corresponding Western blots using Phospho-ALK (Tyr1604) Antibody #3341 (right panel) or a total ALK Rabbit mAb #3333 (left panel), are shown in the bottom figure.
Figure 2: The relationship between protein concentration of phospho or non-phospho lysates and the absorbance at 450 nm is shown. Unstarved Karpas299 cells were cultured (10 6 cells/ml) and lysed with or without addition of phosphatase inhibitor to the lysis buffer (phospho or non-phospho lysate).