FIGURE 1. Mouse brain was cross-linked and disaggregated into a single-cell suspension using a Dounce homogenizer. The chromatin was prepared and digested, and chromatin immunoprecipitations were performed using the indicated ChIP-validated antibodies. Purified DNA was analyzed by quantitative real-time PCR using SimpleChIP ® Mouse GAPDH Intron 2 Primers #8986, SimpleChIP ® Mouse RPL30 Intron 2 Primers #7015, SimpleChIP ® Mouse HoxA1 Promoter Primers #7341, and SimpleChIP ® Mouse HoxD10 Exon 1 Primers #7429. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin (equivalent to 1).
FIGURE 2. Mouse liver was cross-linked and disaggregated into a single-cell suspension using a tissue disaggregator. The chromatin was prepared and digested, and chromatin immunoprecipitations were performed using the indicated ChIP-validated antibodies. Purified DNA was analyzed by quantitative real-time PCR using SimpleChIP ® Mouse GAPDH Intron 2 Primers #8986, SimpleChIP ® Mouse AFM Intron 2 Primers #7269, SimpleChIP ® Mouse HoxA1 Promoter Primers #7341, and SimpleChIP ® Mouse HoxD10 Exon 1 Primers #7429. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin (equivalent to 1).
FIGURE 3. Mouse brain or mouse liver were cross-linked and disaggregated into a single-cell suspension using a Dounce homogenizer or tissue disaggregator, respectively. The chromatin was prepared and digested, and chromatin immunoprecipitations were performed using the indicated ChIP-validated antibodies. DNA was purified and 10 μl was separated by electrophoresis on a 1% agarose gel and stained with ethidium bromide. The majority of chromatin from both brain (lane 1) and liver (lane 2) was digested to 1 to 5 nucleosomes in length (150 to 900 bp).