MAP Kinase, p44/42, phosphorylated (Thr202,Tyr204) Growth Proliferation Marker IHCDetection Kit, BioAssay™

• Supplier: USBiological Life Science
• Catalog: M2352-31
• Applications: IHC
• Description: Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation (1-4). MAP kinases are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK) (5,6). Both kinases are known to weakly autophosphorylate on tyrosine (5). Specificity/Sensitivity: Growth/Proliferation Marker: Phospho-p44/42 MAPK (Thr202/Tyr204) IHC Detection Kit recognizes endogenous levels of p42 and p44 MAP kinase (Erk1 and Erk2) only when phosphorylated at threonine 202 and tyrosine 204 of human Erk, or threonine 183 and tyrosine 185 of rat Erk2. The antibody does not cross-react with the corresponding phosphorylated residues of