Active Arf1 Detection Kit
| Name | Active Arf1 Detection Kit |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 8816 |
| Category | Cellular Assay |
| Prices | $699.00 |
| Sizes | 1 kit (30 reactions) |
| Description | The Active Arf1 Detection Kit provides all reagents necessary for measuring activation of Arf1 GTPase in the cell |
| Supplier Page | Shop |
Product images
Figure 2. The GTP-bound GTPase pull-down process can be divided into 3 steps as shown. Step 1: Mix sample, binding protein, and glutathione resin in the spin cup and incubate at 4ºC to allow GTP-bound GTPase binding to the glutathione resin through GST-linked binding protein. Step 2: Remove unbound proteins by centrifugation. Step 3: Elute glutathione resin-bound GTPase with SDS buffer. The eluted sample can then be analyzed by western blot.