| Description |
PCNA (Proliferating Cell Nuclear Antigen) was previously known as cyclin. PCNA is a nonhistone protein (MW 36kD) found in the nucleus that plays a role in the initiation of cell proliferation by mediating DNA polymerase. PCNA levels are elevated in the S, G2, and M phases of cell mitosis in normal and malignant tissues. PCNA expression has a broad correlation with mitotic activity and can be used as a marker for cell proliferation. PCNA has proven useful for proliferative studies of normal and neoplastic tissues both in vivo and in vitro. This kit uses a biotinylated PCNA monoclonal antibody, thus eliminating the need for a species-specific secondary antibody. As a result, PCNA staining is exceptionally clean and can be performed in tissue and cell samples from most species, including: human, primate, mouse, rabbit, rat, yeast and insect without background caused by crossreactivity with endogeneous immunoglobulins. Streptavidin-peroxidase is used as a signal generator, and DAB as the c
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