Figure 1: Treatment of NKM-1 cells with Human Macrophage Colony Stimulating Factor (hM-CSF) #8929 stimulates tyrosine phosphorylation of M-CSF receptor protein, as detected by PathScan ® Phospho-M-CSF Receptor (panTyr) Sandwich ELISA Kit, but does not affect the level of total M-CSF receptor detected by PathScan ® Total M-CSF Receptor Sandwich ELISA Kit #13032. The absorbance readings at 450 nm are shown in the top figure while corresponding western blots using M-CSF Receptor Antibody #3152 (left panel) and Phospho-M-CSF Receptor (Tyr699) (D10B11) Rabbit mAb #12251 (right panel) are shown in the bottom figure.
Figure 2: The relationship between protein concentration of lysates from untreated and hM-CSF-treated NKM-1 cells and the absorbance at 450 nm as detected by the PathScan ® Phospho-M-CSF Receptor (panTyr) Sandwich ELISA Kit is shown. Unstarved NKM-1 cells (0.5-1.0 x 10 6 ) were treated with Human Macrophage Colony Stimulating Factor (hM-CSF) #8929 (50 ng/ml) for 2-5 min at 37°C and then lysed.