K252a
| Name | K252a |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 12754 |
| Prices | $137.00 |
| Sizes | 100 µg |
| Formula | C27H21N3O5 |
| Molecular Weight | 467.47 g/mol |
| Purity | >99% |
| Description | Originally isolated from the soil fungi Nocardiopisis sp. , K252a is a staurosporine analog that inhibits various protein kinases through competition with the ATP binding site (1,2). K252a is a reversible cell-permeable, potent inhibitor of phosphorylase kinase (IC 50 = 1.7 nM), protein kinase A (PKA) (IC 50 = 140 nM), and protein kinase C (PKC) (IC 50 = 470 nM) (3,4). K252a is also known to inhibit protein kinase G and CaM kinase II (5,6). K252a inhibits nerve growth factor induced Trk activation (IC 50 = 3 nM), as well as downstream signaling (5,7,8). K252a is reported to induce apoptosis and cell cycle arrest through cdc25 and cdc2 inhibition (9). |
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Product images
Western blot analysis of extracts from HeLa cells, serum-starved overnight and untreated or treated with TPA #4174 (200 nM, 15 min) either with or without K252a pretreatment (1 hr) at the indicated concentrations, using Phospho-MARCKS (Ser167/170) (D13E4) XP ® Rabbit mAb #8722 (upper) or MARCKS (D88D11) XP ® Rabbit mAb #5607 (lower).
Western blot analysis of extracts from PC-12 cells, serum-starved overnight and untreated or treated with Human β-Nerve Growth Factor (hβ-NGF) #5221 (100 ng/ml, 5 min) either with or without K252a pretreatment (1 hr) at the indicated concentrations, using Phospho-TrkA (Tyr490) Antibody #9141 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Chemical structure of K252a.