PD98059
| Name | PD98059 |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 9900 |
| Prices | $59.00, $109.00 |
| Sizes | 1.5 mg, 5 mg |
| Formula | C16H13NO3 |
| Molecular Weight | 267.28 g/mol |
| Purity | >99% |
| Description | The MAPK (Erk) kinases MEK1 and MEK2 are dual-specificity protein kinases that play a role in a mitogen activated protein kinase cascade that controls cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of Ser217 and Ser221 within the activation loop of subdomain VIII by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines, and through membrane depolarization and calcium influx (1-4). Constitutively active MEK1/2 can promote transformation of NIH/3T3 cells and differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII. |
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Product images
Western blot analysis of extracts from 293 cells, untreated (-) or NaCl-treated (0.7 M, 30 min; +) and PD98059 pretreatment (indicated concentrations, 1 hr), using Phospho-SEK1/MKK4 (Thr261) Antibody #9151.
Western blot analysis of extracts from COS cells transfected with pCMV-MKK3, untreated (-) or treated with Anisomycin #2222 (20 μg/ml, 30 min; +) and PD98059 pretreatment (indicated concentrations, 1 hr), using Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody #9231.
Western blot analysis of extracts from SK-N-MC cells, untreated (-) or treated with Human Basic Fibroblast Growth Factor (hFGF basic/FGF2) #8910 (100 ng/ml, 30 min; +) and PD98059 pretreatment (indicated concentrations, 1 hr), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101.
