MG-63 cells were incubated at 37°C for 24h with vehicle control (0 µM) and different concentrations of 3-aminobenzamide (3-AB) (ab 141069). Decreased expression of cyclin D1 (ab134175) in MG-63 cells correlates with an increase in 3-aminobenzamide (3-AB) concentration, as described in literature. Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab134175 at 1/500 dilution and ab8226 at 1 µg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.