ab26 staining p53 in U20S cells treated with NSC 146109 hydrochloride (ab142144), by ICC/IF. Increase in p53 expression correlates with increased concentration of NSC 146109 hydrochloride, as described in literature.The cells were incubated at 37°C for 5 hour in media containing different concentrations of ab142144 (NSC 146109 hydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab26 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.