?-Grammotoxin SIA inhibits CaV2.2 channels heterologously expressed in Xenopus oocytes. Left: Time course showing current amplitude in response to 100 ms test pulse to 0 mV delivered every 10 seconds before, during (bar) and after application of 500 nM ?-Grammotoxin SIA (ab141803), which inhibits 60% of N-type currents. The box denoting +100 mV shows the period in which similar pulses to +100 mV were delivered in order to partially relieve the inhibition. Right: The current traces were taken at the indicated positions.