Phorbol 12-myristate 13-acetate (PMA)

• Supplier: Abcam
• Catalog: ab120297
• Cas Number: 16561-29-8
• Pubchem: 27924
• Chemical Name: Phorbol 12-myristate 13-acetate
• Formula: C36H56O8
• Molecular Weight: 616.83
• Purity: > 99%
• Description: PKC activator

Product images

Serum starved HeLa cells were incubated at 37°C for 60 minutes with vehicle control (0 µM) and different concentrations of phorbol 12-myristate 13-acetate (PMA) (ab120297) in DMSO. Increased expression of PKC mu (phospho S916) (ab81218) correlates with an increase in phorbol 12-myristate 13-acetate (PMA) concentration, as described in literature.Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab81218 at 1 µg/ml and ab8227 at 1 µg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

Sandwich ELISA - TNF alpha Human ELISA Kit (ab100654) TNFa detected in supernatants from control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), and PMA with the addition of 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. PBMCs were stimulated for 48 hours with 2 % PHA-M (LifeTechnologies). Results shown after background signal was subtracted (duplicates +/- SD).

Sandwich ELISA - IL-6 (Interleukin-6) Mouse ELISA Kit (ab100712).IL-6 detected in supernatants from RAW 246.7 control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), or 24 hours with PMA and 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. Results shown after background signal was subtracted (duplicates +/- SD).

Sandwich ELISA - IL-1ra (Interleukin-1ra) Mouse ELISA Kit (ab113348)IL-1Ra detected in supernatants from RAW 246.7 control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), or 24 hours with PMA and 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. Results shown after background signal was subtracted (duplicates +/- SD).

Sandwich ELISA - IFN gamma Human ELISA Kit (ab46025)Jurkat were stimulated for 48 hours with 50 ng x mL-1 of PMA (ab120297) and 1 uM Ionomycin (ab120116) and PBMCs were stimulated for 48 hours with 2 % PHA-M (LifeTechnologies). Cell free supernatants were tested, showing results after background signal was subtracted (duplicates +/- SD).

Product References

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