(R,S)-CHPG
| Name | (R,S)-CHPG |
|---|---|
| Supplier | Abcam |
| Catalog | ab120039 |
| Cas Number | 170846-74-9 |
| Pubchem | 3645780 |
| Chemical Name | (R,S)-2-Amino-2-(2-chloro-5-hydroxyphenyl)acetic acid |
| Formula | C8H8NO3Cl |
| Molecular Weight | 201.61 |
| Purity | > 98% |
| Description | Selective mGlu5 agonist |
| Supplier Page | Shop |
Product images
SK-N-SH cells were incubated at 37°C for 30 minutes with vehicle control (0 µM) and different concentrations of (R,S)-CHPG (ab120039). Increased expression of CREB (phospho S133) in SK-N-SH cells correlates with an increase in (R,S)-CHPG concentration, as described in literature.Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab32096 at 1/500 dilution and ab32515 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
Product References
Memory for fear extinction requires mGluR5-mediated activation of infralimbic - Memory for fear extinction requires mGluR5-mediated activation of infralimbic
Fontanez-Nuin DE, Santini E, Quirk GJ, Porter JT. Cereb Cortex. 2011 Mar;21(3):727-35.
Group I mGluR agonist-evoked long-term potentiation in hippocampal oriens - Group I mGluR agonist-evoked long-term potentiation in hippocampal oriens
Le Duigou C, Kullmann DM. J Neurosci. 2011 Apr 13;31(15):5777-81.
Interoceptive effects of alcohol require mGlu5 receptor activity in the nucleus - Interoceptive effects of alcohol require mGlu5 receptor activity in the nucleus
Besheer J, Grondin JJ, Salling MC, Spanos M, Stevenson RA, Hodge CW. J Neurosci. 2009 Jul 29;29(30):9582-91.