ab65783 staining NR2B in SKNSH cells treated with GBR 12909 dihydrochloride (ab120607), by ICC/IF. Decrease in NR2B expression correlates with increased concentration of GBR 12909 dihydrochloride, as described in literature.The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120607 (GBR 12909 dihydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab65783 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

ab65783 staining NR2B in SKNSH cells treated with GBR 12909 dihydrochloride (ab120607), by ICC/IF. Decrease in NR2B expression correlates with increased concentration of GBR 12909 dihydrochloride, as described in literature.The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120607 (GBR 12909 dihydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab65783 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.


Go to product page


Image may be subject to copyright.