Figure 1. Constitutive phosphorylation of Src at Tyr416 in Calu-3 cells as detected by the PathScan® Phospho-Src (Tyr416) Sandwich ELISA Kit #7953. Treatment of lysate with λ-phosphatase significantly reduces Src phosphorylation but does not alter the levels of total Src protein as detected by the PathScan® Total Src Sandwich ELISA kit #7984. Absorbance at 450 nm is shown in the top figure while the corresponding western blots using Src (L4A1) Mouse mAb #2110 (left) or Phospho-Src Family (Tyr416) (100F9) Rabbit mAb #2113 (right) are shown in the bottom figure.

Figure 1. Constitutive phosphorylation of Src at Tyr416 in Calu-3 cells as detected by the PathScan® Phospho-Src (Tyr416) Sandwich ELISA Kit #7953. Treatment of lysate with λ-phosphatase significantly reduces Src phosphorylation but does not alter the levels of total Src protein as detected by the PathScan® Total Src Sandwich ELISA kit #7984. Absorbance at 450 nm is shown in the top figure while the corresponding western blots using Src (L4A1) Mouse mAb #2110 (left) or Phospho-Src Family (Tyr416) (100F9) Rabbit mAb #2113 (right) are shown in the bottom figure.


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