FOX-NY cells were incubated at 37°C for 30 minutes with vehicle control (0 µM) and different concentrations of nifuroxazide (ab120951). Decreased expression of Stat3 (phospho Y705) (ab76315) in FOX-NY cells correlates with an increase in nifuroxazide concentration, as described in literature.Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab76315 at 1/20000 dilution and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

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