PD 153035 inhibits EGF receptor activation in 3T3-L1 cells. Cells were serum starved for 3 h and preincubated with different concentrations of PD 153035 (ab141839) for 2 h. The cells were then stimulated with 200 ng/ml EGF for 10 minutes. Cell proteins were resolved by SDS-PAGE and probed with anti-phospho-EGFr (upper panel) and with anti-phospho ERK1/2 (lower panel).

PD 153035 inhibits EGF receptor activation in 3T3-L1 cells. Cells were serum starved for 3 h and preincubated with different concentrations of PD 153035 (ab141839) for 2 h. The cells were then stimulated with 200 ng/ml EGF for 10 minutes. Cell proteins were resolved by SDS-PAGE and probed with anti-phospho-EGFr (upper panel) and with anti-phospho ERK1/2 (lower panel).


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