ab1223 staining transferrin in HeLa cells treated with sertraline hydrochloride (ab141068), by ICC/IF. Decrease in transferrin expression correlates with increased concentration of sertraline hydrochloride, as described in literature.The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab141068 (sertraline hydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1223 (1/500 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

ab1223 staining transferrin in HeLa cells treated with sertraline hydrochloride (ab141068), by ICC/IF. Decrease in transferrin expression correlates with increased concentration of sertraline hydrochloride, as described in literature.The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab141068 (sertraline hydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1223 (1/500 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.


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