ab9969 staining IL10 in Raw 264.7 cells treated with spermine (ab120051), by ICC/IF. Increase in IL10 expression correlates with increased concentration of spermine, as described in literature.The cells were incubated at 37°C for 6h in media containing different concentrations of ab120051 (spermine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab9969 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

ab9969 staining IL10 in Raw 264.7 cells treated with spermine (ab120051), by ICC/IF. Increase in IL10 expression correlates with increased concentration of spermine, as described in literature.The cells were incubated at 37°C for 6h in media containing different concentrations of ab120051 (spermine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab9969 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.


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