Angiotensin III induces MAPK activation in IEC-6 cells similar to Angiotensin II. Cells were incubated in serum depleted medium for 2 h and then stimulated with 0.1 µM and 1 µM Angiotensin III (ab141805) for 10 min or with 0.1 µM and 1 µM Angiotensin II (ab120183) for comparison. Cell proteins were resolved by SDS-PAGE and probed with anti-phospo-ERK1/2.

Angiotensin III induces MAPK activation in IEC-6 cells similar to Angiotensin II. Cells were incubated in serum depleted medium for 2 h and then stimulated with 0.1 µM and 1 µM Angiotensin III (ab141805) for 10 min or with 0.1 µM and 1 µM Angiotensin II (ab120183) for comparison. Cell proteins were resolved by SDS-PAGE and probed with anti-phospo-ERK1/2.


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