Active human Glutaminase full length protein
| Name | Active human Glutaminase full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab198065 |
| Category | Protein |
| Prices | $1,220.00 |
| Sizes | 20 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Tag/Conjugation | His tag N-Terminus |
| Purity | >= 74 % by SDS-PAGE. |
| Bioactivity | Specific Activity: ≥ 25 nmol/min/µg Assay Conditions: The reaction mixture contained 100 mM Tris, pH 8.6, 150 mM K 2 HPO 4 , 25 mM L-glutamine, 2 mM NAD+, 4 units of GDH, and various amounts of ab198065 in a volume of 200 μl. Reactions were initiated with the addition of ab198065, and the increase in NADH absorbance at 340 nm was continuously monitored for 7 minutes at 37°C. Molar extinction coefficient of NADH is 6,220 M -1cm-1 . |
| SwissProt/Accession | O94925 |
| Gene | GLS |
| Residue | 17 to 669 |
| Sequence | M HHHHHH SPAGVSATLRRAQPLVTLCRRPRGGGRPAAGPAAAARLH PWWGGGGWP AEPLARGLSSSPSEILQELGKGSTHPQPGVSPPAAPAAP GPKDGPGETDAF GNSEGKE LVASGENKIKQGLLPSLEDLLFYTIAE GQEKIPVHKFITALKSTGLRTSDP RLKECMDML RLTLQTTSDGVML DKDLFKKCVQSNIVLLTQAFRRKFVIPDFMSFTSHIDE LYESAKKQS GGKVADYIPQLAKFSPDLWGVSVCTVDGQRHSTGDTKVPFCLQSCVKPL KY AIAVNDL GTEYVHRYVGKEPSGLRFNKLFLNEDDKPHNPMVNAG AIVVTSLIKQGVNN AEKFDYV MQFLNKMAGNEYVGFSNATFQSERE SGDRNFAIGYYLKEKKCFPEGTDMVG ILDFYFQ LCSIEVTCESASV MAATLANGGFCPITGERVLSPEAVRNTLSLMHSCGMYD FSGQFAFH VGLPAKSGVAGGILLVVPNVMGMMCWSPPLDKMGNSVKGIHFCHDLVSLC N FHNYDN LRHFAKKLDPRREGGDQRVKSVINLLFAAYTGDVSALRR FALSAMDMEQRD YDSRTAL HVAAAEGHVEVVKFLLEACKVNPFPKD RWNNTPMDEALHFGHHDVFKILQE YQVQYTP QGDSDNGKENQTVHK NLDGLL |
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