All lanes : Anti-ENT1 antibody ( ab48607 ) at 2 µg/ml Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue ( ab29431 ) Lane 2 : Brain (Human) Tissue Lysate - adult normal tissue ( ab29466 ) Lane 3 : Heart (Human) Tissue Lysate - adult normal tissue ( ab29431 ) with Human ENT1 peptide (ab48606) at 2 µg/ml Lane 4 : Brain (Human) Tissue Lysate - adult normal tissue ( ab29466 ) with Human ENT1 peptide (ab48606) at 2 µg/ml Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Performed under reducing conditions. Predicted band size : 50 kDa Observed band size : 55 kDa ( why is the actual band size different from the predicted? ) Additional bands at : 36 kDa,78 kDa. We are unsure as to the identity of these extra bands. Exposure time : 4 minutes