Recombinant MLL4 Complex
| Name | Recombinant MLL4 Complex |
|---|---|
| Supplier | ActiveMotif |
| Catalog | 31499 |
| Prices | $415.00, $3,500.00 |
| Sizes | 20 µg, 1 mg |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Gene | KMT2B |
| Description | Recombinant MLL4 Complex contains amino acids 2551 - 2715 of the human MLL4 protein (accession number NP_055542.1) with N-terminal GST tag and MW= 47.6 kDa; full length human WDR5 (GenBank Accession No.NM_017588), with N-terminal 6×His tag and MW= 35 kDa; full length human ASH2L (GenBank Accession No. NM_001105214), N-terminal 6×His tag and MW= 61 kDa; full length human RbBP5 (GenBank Accession No. NM_005057), N-terminal 6×His-tag and MW= 60 kDa, and full length human DPY30 (GenBank Accession No. NM_032574), N-terminal 6×His-tag and MW=12 kDa, all individually expressed in E. coli cells |
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Product images
Recombinant MLL4 Complex gel MLL4 Complex was run on an 10% SDS-PAGE gel and stained with Coomassie blue.
Recombinant MLL4 Complex HTRF activity assay 3. 3 μM H3K4me0 (1-21aa) peptide was incubated with MLL4 Complex in reaction buffer for 3 hour at room temperature. MLL4 Complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.
Recombinant MLL4 Complex MALDI-TOF activity assay 3. 3 uM H3K4me0 peptide was incubated with 20 nM MLL4 Complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3. 3 uM H3K4me0 peptide was used as negative control. Catalytic Ability: 50 turnovers/ enzyme molecule