Densitometry of protein isoforms visualised by 2-DE. The triangle indicates the theoretical MW and pI of the protein.
1D gel data: Lane 1 ab83685 Lane 2 ab83685 treated with PNGase F to remove potential N-linked glycans Lane 4 ab83685 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 5 µg protein loaded per lane; Deep Purple™ stained. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Faint bands in lane 2 and lane 3 are glycosidase enzymes.
2D gel data: A sample of ab83685 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 µg protein loaded per lane; Deep Purple™ stained. Spot train indicates presence of multiple isoforms of ab83685. Spots within the spot train were cut from the gel and identified as IL-3 by protein mass fingerprinting.