Active human Activin A full length protein
| Name | Active human Activin A full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab50051 |
| Category | Protein |
| Prices | $110.00, $257.00, $316.00 |
| Sizes | 2 µg, 5 µg, 10 µg |
| Applications | Cell differentiation SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Purity | > 95 % by SDS-PAGE. Endotoxin level is less than 0.1 ng per µg (1EU/µg). |
| Bioactivity | Biological Activity : The ED 50 as determined by its ability to inhibit the proliferation of murine MPC-11 cells is < 2 ng/ml, corresponding to a specific activity of > 5 x 10 5 units/mg. |
| Endotoxin | < 0.100 Eu/µg |
| SwissProt/Accession | P08476 |
| Gene | INHBA |
| Residue | 311 to 426 |
| Sequence | GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS |
| Supplier Page | Shop |
Product images
Human embryonic stem cells (HUES4) were differentiated as monolayer cultures on matrigel coated dishes using a protocol described in Kroon et al, Nat Biotechnol. (2008). Activin A (ab50051) and Wnt3a ( ab81484 ) were added to cultures to direct differentiation towards definitive endoderm. This image was taken 3 days after induction, where the definitive endoderm marker FOXA2 ( ab40874 in green) is abundant. Dapi staining is shown in blue. Magnification: 200x. Negative controls of fluorescently conjugated secondary antibodies only showed no staining.