Active human Met (c-Met) recombinant protein fragment
| Name | Active human Met (c-Met) recombinant protein fragment |
|---|---|
| Supplier | Abcam |
| Catalog | ab42612 |
| Category | Protein |
| Prices | $340.00 |
| Sizes | 10 µg |
| Applications | B/N |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | proprietary tag N-Terminus |
| Bioactivity | 40 U/µg. One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na-orthovanadate, 0.1 mM ATP, 30 µg/ml Poly (Glu:Tyr)4:1 substrate and 4 µg/ml recombinant c-Met. |
| SwissProt/Accession | P08581 |
| Gene | MET |
| Residue | 956 to 1390 |
| Sequence | Belongs to the protein kinase superfamily. Tyr protein kinase family. Contains 3 IPT/TIG domains. Contains 1 protein kinase domain. Contains 1 Sema domain. |
| Supplier Page | Shop |
Product images
10% SDS-PAGE Gel, Stained with Coomassie Blue, 4μg of ab42612 loaded onto Gel.
Specific Activity: 64pmol/min/ug. cMet was incubated with 2uM substrate (Tyr2 peptide) for 1h at 30°C in a buffer containing 50mM HEPES (ph7.5), 10mM MgCl2, 1mM EDTA, 0.01% BRIJ-35 and 200uM ATP. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT.