Active human MMP2 full length protein

• Supplier: Abcam
• Catalog: ab125181
• Category: Protein
• Prices: $192.00
• Sizes: 10 µg
• Applications: FA SDS-PAGE
• Species Reactivities: Human
• Nature: Recombinant
• Source: HEK 293 cells
• Tag/Conjugation: His tag C-Terminus
• Purity: > 95 % by SDS-PAGE. ab125181 is purified by proprietary chromatographic techniques.
• Bioactivity: The activity was measured by its ability to cleave the colorimetric peptide substrate, Mca-PLGL-DpaAR-NH2, The specific activity is > 1,000 pmoles/min/µg.
• SwissProt/Accession: P08253
• Gene: MMP2
• Residue: 30 to 660
• Sequence: Belongs to the peptidase M10A family. Contains 3 fibronectin type-II domains. Contains 4 hemopexin-like domains.

Product images

Detection of gelatinolytic activity of ab125181 MMP2 protein. 10 ng of ab125181 in 25 µl of 25 mM Tris-glycine buffer (pH 6.8) was mixed with 2x SDS-sample buffer (without ß-mercaptoethanol) and incubated at 37°C for 10 minutes. Samples were loaded on a 10% acrylamide-Tris-glycine gel containing 0.1% gelatin and electrophoresed using Tris-glycine (pH 8.3) with 0.1% SDS. Electrophoresis was performed at 90V for 2 hours. The gel was washed 3 times in 40-50 ml of 2.5% Triton X-100 for 15 minutes. The gel was immersed in 40-50 ml of 50 mM Tris-glycine (pH 7.5), 100 mM NaCl, 4 mM CaCl 2 and 0.02% Triton X-100 and incubated at 37°C for 16 hours. For detection of the gelatinolytic activity, the gel was stained with 0.05% Commassie Blue. ab125181 produced a prominent gelatinolytic band corresponding to a molecular mass of ~ 68 kDa. See Abreview