All lanes : Anti-N Cadherin antibody ( ab18203 ) at 1 µg/ml Lane 1 : Rat Brain Lysate Lane 2 : Mouse Brain Lysate Lane 3 : Rat Brain Lysate with Human N Cadherin peptide (ab18620) at 1 µg/ml Lane 4 : Mouse Brain Lysate with Human N Cadherin peptide (ab18620) at 1 µg/ml Lysates/proteins at 20 µg per lane. Secondary Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution Performed under reducing conditions. Predicted band size : 135 kDa Observed band size : 135 kDa Additional bands at : 50 kDa (possible truncated form).