Name |
Native Chlamydia trachomatis LGV Type-2 EB Protein
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Supplier |
Creative Biomart
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Catalog |
CTLGV2EB-359C
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Category |
Protein
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Species Reactivities |
Chlamydia trachomatis
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Nature |
Native
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Source |
Mouse L Cells
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Purity |
Mouse L cells are infected with C. trachomatis elementary bodies. Optimally infected cells are harvested and disrupted by sonication in PBS. Cellular debris is removed using centrifugation.
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Bioactivity |
Inactivated using gamma radiation. Effective primarily by damaging chlamydial genetic material. Effectiveness of inactivation is tested by inoculating an L cell monolayer with antigen. The culture is manipulated using the original optimal culture conditions used to manufacture the antigen. The culture is monitored for cytopathic effect for 5 days. If no signs of infection are observed the culture is passaged into a fresh monolayer. The second passage is monitored for a further 5 day period. If no cytopathic effect is observed in either passage the antigen is considered inactivated. Result: No growth detected.
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Description |
Native Chlamydia trachomatis LGV Type-2 EB (Elementary Bodies) Antigen Protein is prified from Mouse L Cells.
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Supplier Page |
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