Active human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha full length protein
| Name | Active human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab196129 |
| Category | Protein |
| Prices | $837.00 |
| Sizes | 20 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | DDDDK tag N-Terminus |
| Purity | > 55 % by SDS-PAGE. |
| Bioactivity | Specific Activity: 800 pmol/min/μg A unit of Active human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha full length protein activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C. |
| SwissProt/Accession | P42336 |
| Gene | PIK3CA |
| Residue | 1 to 1068 |
| Sequence | MPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLITIKHELF KEARKYPLHQLLQDESSYIFVSVTQEAEREEFFDETRRLCDLRLFQPFLK VIEPVGNREEKILNREIGFAIGMPVCEFDMVKDPEVQDFRRNILNVCKEA VDLRDLNSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSP NNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKY ILKVCGCDEYFLEKYPLSQYKYIRSCIMLGRMPNLMLMAKESLYSQLPMD CFTMPSYSRRISTATPYMNGETSTKSLWVINSALRIKILCATYVNVNIRD IDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAA RLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPH GLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSV SREAGFSYSHAGLSNRLARDNELRENDKEQLKAISTRDPLSEITKQEKDF LWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAME LLDCNYPDPMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNLLV RFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLK HLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDFMDALQG FLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNEII FKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEV VRNSHTIMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEIYDAAIDLFTRS CAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRE RVPFVLTQDFLIVISKGAQECTKTREFERFQ |
| Supplier Page | Shop |
Product images
10% SDS-PAGE with Coomassie stain. Lane 1: 5 µg Active human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha full length protein (ab196129) Lane 2: Protein marker
Specific activity of ab196129. A 25 μl kinase reaction is conducted in a buffer containing 40 mM Tris-HCl (pH 7.4), 20 mM MgCl 2 , 100 uM ATP, 200 μM PI:PS substrate and Active human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha full length protein (ab196129) for 15 minutes at 30°C.