Active human PKLR protein fragment
| Name | Active human PKLR protein fragment |
|---|---|
| Supplier | Abcam |
| Catalog | ab100984 |
| Category | Protein |
| Prices | $308.00, $1,234.00 |
| Sizes | 50 µg, 250 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Tag/Conjugation | His tag N-Terminus |
| Purity | > 90 % by SDS-PAGE. ab100984 is purified using conventional chromatography techniques. |
| Bioactivity | Specific activity: >0.1 unit/mg. One unit will form 1.0 umol of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37°C. Activity Assay: Prepare a 1.45 ml reaction mixture into a suitable container. - Reaction mixture: 100mM Tris-HCl pH 7.5, 7.6 mM ADP, 15 mM MgCl2, 74 mM KCl, 0.2 mM Beta-NADH, 5.2 mM PEP, 0.025 units Recombinant LDHA protein ( ab93699 ) Add 50ul of recombinant PKLR protein solution with various concentrations (0.5ug, 1ug) Read the decrease in A340nm in kinetic mode for 10 minutes. |
| SwissProt/Accession | P30613 |
| Gene | PKLR |
| Residue | 47 to 574 |
| Sequence | MGSSHHHHHHSSGLVPRGSHMLTQELGTAFFQQQQLPAAMADTFLEHLCL LDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHE YHAESIANVREAVESFAGSPLSYRPVAIALDTKGPEIRTGILQGGPESEV ELVKGSQVLVTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLIS LVVQKIGPEGLVTQVENGGVLGSRKGVNLPGAQVDLPGLSEQDVRDLRFG VEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDE ILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLE SMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIA REAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLT TTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIW ADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS |
| Supplier Page | Shop |
Product images
15% SDS-PAGE analysis of 3µg ab100984.