Active human VEGFB full length protein

Name Active human VEGFB full length protein
Supplier Abcam
Catalog ab87052
Category Protein
Prices $410.00
Sizes 50 µg
Applications FA SDS-PAGE
Species Reactivities Human
Nature Recombinant
Source E. coli
Purity > 90 % by SDS-PAGE. ab87052 was purified using conventional chromatography techniques
Bioactivity Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is < 1ng/ml. Activity Assay: Cell line: NIH-3T3 (Mus musculus/Mouse embryonic fibroblast) Maintenance Condition: RPMI 1640 containing 10% FBS Assay medium: serum free RPMI 1640 Cell density: 2 x 104 cells/well (96 well plate, final volume 100ul) Incubation time : 24 hr (after sample treatment) Concentration range: 10pg/ml - 10ug/ml Detection method : BrdU assay
Endotoxin < 1.000 Eu/µg
SwissProt/Accession P49765
Gene VEGFB
Residue 22 to 207
Sequence MGSSHHHHHH SSGLVPRGSH MPVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP STTSALTPGP AAAAADAAAS SVAKGGA
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