Recombinant BPTF / FALZ (2791-2911) protein

• Supplier: ActiveMotif
• Catalog: 31447
• Category: Protein
• Prices: $395.00, $2,600.00
• Sizes: 100 μg, 1 mg
• Species Reactivities: Human
• Nature: Recombinant
• Source: E. coli
• Gene: BPTF
• Description: Recombinant BPTF / FALZ (2791-2911) protein that includes amino acids 2791-2911 of human FALZ protein (accession number NP_872579.2) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with a molecular weight of 20.3 kDa

Product images

Recombinant BPTF / FALZ (2791-2911) protein gel. BPTF / FALZ (2791-2911) protein was run on an SDS-PAGE gel and stained with Coomassie blue.

Recombinant BPTF / FALZ (2791-2911) activity using AlphaScreen. BPTF / FALZ (2791-2911) titration and inhibition were assessed using an AlphaScreen® assay. Titration curves were generated to show signal response in the presence of modified peptide substrate at increasing protein concentrations. An IC50 dose response assessment of reference compound JQ1 is also shown. This data was generated and kindly provided courtesy of ChemPartner.

HTRF Assay for Recombinant BPTF / FALZ (2791-2911) activity. 3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BPTF / FALZ (2791-2911) protein in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products.