Recombinant BRDT (257-382), GST-tag

• Supplier: ActiveMotif
• Catalog: 31483
• Prices: $395.00, $2,900.00
• Sizes: 100 µg, 1 mg
• Species Reactivities: Human
• Nature: Recombinant
• Source: E. coli
• Gene: BRDT
• Description: The peptide corresponding to amino acids 257-382 that contains the second bromodomain sequences of BRDT (accession number NP_997072.1) was expressed in E. coli and contains an N-terminal GST tag with an observed molecular weight of 42.8 kDa

Product images

Recombinant BRDT (257-382), GST-tag protein gel. BRDT (257-382), GST-tag protein was run on an SDS-PAGE gel and stained with Coomassie blue.

Recombinant BRDT (257-382) HTRF activity assay. 3. 3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BRDT (257-382) protein in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.

Recombinant BRDT (257-382) HTRF activity assay. 3. 3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BRDT (257-382) protein in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.