Recombinant SMARCA2 / BRM (1367-1511), GST-tag protein gel. SMARCA2 / BRM (1367-1511), GST-tag protein was run on an SDS-PAGE gel and stained with Coomassie blue.
Recombinant SMARCA2 / BRM (1367-1511), GST-tag activity using AlphaLISA. SMARCA2 / BRM (1367-1511), GST-tag was used in an AlphaLISA assay to determine enzyme linearity. This data was generated and kindly provided courtesy of ChemPartner.
Recombinant SMARCA2 / BRM (1367-1511) HTRF activity assay 3. 3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with SMARCA2 / BRM (1367-1511) protein in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.